NCK Family
Aliases: NCK tyrosine kinase, NCK adaptor protein 1, melanoma NCK protein, SH2/SH3 adaptor protein NCK-alpha, non-catalytic region of tyrosine kinase, NCK, NCK1, MGC12668, NCKalpha
Processes & Diseases
Processes involved: adhesion
Pfam Domain Structure
From NCK1 (human)
Members of the NCK Family
| 1: NCK1 » | Human | Mouse | Rat | Chicken | Fly | Worm |
| CMKB | NCK1 | Nck1 | Nck1 | - | dock | - |
| Entrez Gene | 4690 | 17973 | 300955 | - | 33262 | - |
| Uniprot | NCK1_HUMAN | - | - | - | - | - |
| OMIM | 600508 | - | - | - | - | - |
| Reagents | - | 2 available | - | - | - | - |
| Results | 3 available | - | - | - | 2 available | - |
| 2: NCK2 » | Human | Mouse | Rat | Chicken | Fly | Worm |
| CMKB | NCK2 | Nck2 | Nck2 | NCK2 | - | nck-1 |
| Entrez Gene | 8440 | 17974 | 316369 | 418727 | - | 180690 |
| Uniprot | NCK2_HUMAN | - | - | - | - | - |
| OMIM | 604930 | - | - | - | - | - |
| Reagents | - | 2 available | - | - | - | - |
| Results | 2 available | - | - | - | - | - |
Family Summary
NCK1 and NCK2 are adaptor proteins involved in the integrin signalling pathway from the extracellular space into the cell and interact with the receptor tyrosine kinases to regulate survival, proliferation and cell shape.
References
- Baumgartner M, Sillman AL, Blackwood EM, Srivastava J, Madson N, Schilling JW, Wright JH, Barber DL. The Nck-interacting kinase phosphorylates ERM proteins for formation of lamellipodium by growth factors. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:13391-6 (PubMed)
- Blasutig IM, New LA, Thanabalasuriar A, Dayarathna TK, Goudreault M, Quaggin SE, Li SS, Gruenheid S, Jones N, Pawson T. Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization. Mol. Cell. Biol. 2008; 28:2035-46 (PubMed)
- Buday L, Wunderlich L, Tamás P. The Nck family of adapter proteins: regulators of actin cytoskeleton. Cell. Signal. 2002; 14:723-31 (PubMed)
- Li W, Fan J, Woodley DT. Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 2001; 20:6403-17 (PubMed)
- Rivera GM, Antoku S, Gelkop S, Shin NY, Hanks SK, Pawson T, Mayer BJ. Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:9536-41 (PubMed)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
NCK1 Aliases: NCK, NCKalpha Entrez Gene: 4690 siRNA catalog |
NCK adaptor protein 1 mRNA: NM_006153 Library: MAR Classification: adaptor |
Final bin: Accelerated - MC SMARTpool bin: Accelerated Avg area: 0.27 Avg Alamar: 1.04 Knockdown %: not done |
ERBB2: Accelerated |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
NCK1 Aliases: NCK, NCKalpha Entrez Gene: 4690 siRNA catalog |
NCK adaptor protein 1 mRNA: NM_006153 Library: MAR |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00028065 | NCK1 | CYTOPLASMIC PROTEIN NCK1. | 1 | 1 | 0 | 0 | 0.00 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
dock Flybase Entrez CMCKB |
dreadlocks |
Mig: 118.4 Non-Mig: 82.1 |
1.44210 | 142879_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG3727 Entrez Gene: 33262 |
dreadlocks |
10.938 1.029 |
0.999 | 1.043 |
Actin: 1.063 Area: 0.879 Ratio: 1.209 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
NCK2 Aliases: GRB4, NCKbeta Entrez Gene: 8440 siRNA catalog |
NCK adaptor protein 2 mRNA: NM_003581 Library: MAR Classification: adaptor Focal Adhesion Related: Yes |
Final bin: Low Alamar - MC SMARTpool bin: Low Alamar Avg area: 3.67 Avg Alamar: 0.59 Knockdown %: not done |
ERBB2: No Change BCL2: Low Alamar |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
NCK2 Aliases: GRB4, NCKbeta Entrez Gene: 8440 siRNA catalog |
NCK adaptor protein 2 mRNA: NM_003581 Library: MAR |
 Link |
 Link |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| NCK1 | mouse | Nck1 / Nck2 - embryonic fibroblasts | - |
Defects in cell motility and organization of the lamellipodial actin network. | completed |
-
|
-
|
Bladt et al. |
| NCK1 | mouse | Nck1 / Nck2 | - |
Nck genes have broad and overlapping expression pattern and mice deficient in either Nck1 or Nck2 are viable, whereas inactivation of both resulted in profound defects in mesoderm-derived notochord and embryonic lethality at day 9.5. | completed |
-
|
-
|
Bladt et al. |
| NCK2 | mouse | Nck1 / Nck2 - embryonic fibroblasts | - |
Defects in cell motility and organization of the lamellipodial actin network. | completed |
-
|
-
|
Bladt et al. |
| NCK2 | mouse | Nck1 / Nck2 | - |
Nck genes have broad and overlapping expression pattern and mice deficient in either Nck1 or Nck2 are viable, whereas inactivation of both resulted in profound defects in mesoderm-derived notochord and embryonic lethality at day 9.5. | completed |
-
|
-
|
Bladt et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
The primary classification of CMKB families is the homeomorphic family, whose members are both homologous (evolved from a common ancestor) and homeomorphic (sharing full-length sequence similarity and a common domain architecture).