CaMKII Family
Aliases: calcium/calmodulin-dependent protein kinase IIA, CaMK-II alpha subunit, CaM-kinase II alpha chain, CaM kinase II alpha subunit, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase type II alpha chain, CAMKA, CAMK2A, KIAA0968
Pfam Domain Structure
From CAMK2A (human)
Members of the CaMKII Family
| 1: CAMK2A » | Human | Mouse | Rat | Chicken | Fly | Worm |
| CMKB | CAMK2A | Camk2a | Camk2a | CAMK2A | - | - |
| Entrez Gene | 815 | 12322 | 25400 | 374175 | - | - |
| Uniprot | KCC2A_HUMAN | KCC2A_MOUSE | KCC2A_RAT | - | - | - |
| OMIM | 114078 | - | - | - | - | - |
| Reagents | - | - | - | - | - | - |
| Results | 2 available | - | - | - | - | - |
| 2: CAMK2B » | Human | Mouse | Rat | Chicken | Fly | Worm |
| CMKB | CAMK2B | Camk2b | Camk2b | CAMK2B | - | - |
| Entrez Gene | 816 | 12323 | 24245 | 374174 | - | - |
| Uniprot | KCC2B_HUMAN | KCC2B_MOUSE | KCC2B_RAT | - | - | - |
| OMIM | 607707 | - | - | - | - | - |
| Reagents | - | - | - | - | - | - |
| Results | 2 available | - | - | - | - | - |
| 3: CAMK2D » | Human | Mouse | Rat | Chicken | Fly | Worm |
| CMKB | CAMK2D | Camk2d | Camk2d | CAMK2D | CaMKII | unc-43 |
| Entrez Gene | 817 | 108058 | 24246 | 422688 | 43828 | 177921 |
| Uniprot | KCC2D_HUMAN | KCC2D_MOUSE | KCC2D_RAT | KCC2D_CHICK | KCC2A_DROME | - |
| OMIM | 607708 | - | - | - | - | - |
| Reagents | - | - | - | - | - | - |
| Results | 3 available | - | - | - | 2 available | - |
| 4: CAMK2G » | Human | Mouse | Rat | Chicken | Fly | Worm |
| CMKB | CAMK2G | Camk2g | Camk2g | CAMK2G | - | - |
| Entrez Gene | 818 | 12325 | 171140 | 423737 | - | - |
| Uniprot | KCC2G_HUMAN | KCC2G_MOUSE | KCC2G_RAT | - | - | - |
| OMIM | 602123 | - | - | - | - | - |
| Reagents | - | - | - | - | - | - |
| Results | 2 available | 1 available | - | - | - | - |
Family Summary
The Ca2+/calmodulin-dependent protein kinase, CaMK-II, belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The CamKII Family consists of four different chains: alpha, beta, gamma, and delta. CAMKII has been linked to cell migration, cell cycle control, cell polarity and cytoskeletal behaviors in non-neuronal cells.
References
- Colomer J, Means AR. Physiological roles of the Ca2+/CaM-dependent protein kinase cascade in health and disease. Subcell. Biochem. 2007; 45:169-214 (PubMed)
- Griffith LC. Regulation of calcium/calmodulin-dependent protein kinase II activation by intramolecular and intermolecular interactions. J. Neurosci. 2004; 24:8394-8 (PubMed)
- Lantsman K, Tombes RM. CaMK-II oligomerization potential determined using CFP/YFP FRET. Biochim. Biophys. Acta 2005; 1746:45-54 (PubMed)
- Liu X, Yao M, Li N, Wang C, Zheng Y, Cao X. CaMKII promotes TLR-triggered proinflammatory cytokine and type I interferon production by directly binding and activating TAK1 and IRF3 in macrophages. Blood 2008; 112:4961-70 (PubMed)
- Pitt GS. Calmodulin and CaMKII as molecular switches for cardiac ion channels. Cardiovasc. Res. 2007; 73:641-7 (PubMed)
- Seward ME, Easley CA, McLeod JJ, Myers AL, Tombes RM. Flightless-I, a gelsolin family member and transcriptional regulator, preferentially binds directly to activated cytosolic CaMK-II. FEBS Lett. 2008; 582:2489-95 (PubMed)
- Si J, Mueller L, Collins SJ. CaMKII regulates retinoic acid receptor transcriptional activity and the differentiation of myeloid leukemia cells. J. Clin. Invest. 2007; 117:1412-21 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| CAMK2B | none | binding partner | beta subunit of CaMK-II / delta subunit of CaMK-II | Ectopic expression in NIH 3T3 cells | 9 CaMK-II binding proteins identified by ectopic expression of FLAG-tagged CaMK-II splice variants | Horwitz | Seward et al. |
| CAMK2D | none | binding partner | beta subunit of CaMK-II / delta subunit of CaMK-II | Ectopic expression in NIH 3T3 cells | 9 CaMK-II binding proteins identified by ectopic expression of FLAG-tagged CaMK-II splice variants | Horwitz | Seward et al. |
Discovery» PyMT invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Camk2g Entrez Gene: 12325 |
mRNA: W71216 UniGene: Mm.235182 |
Calcium/calmodulin-dependent protein kinase II gamma | 4.35 |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
CAMK2A Aliases: CAMKA Entrez Gene: 815 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha mRNA: NM_015981 Library: Kinase Classification: Ser/Thr Kinase |
Final bin: Discordant SMARTpool bin: Impaired Avg area: 2.40 Avg Alamar: 0.84 Knockdown %: 90-100 |
ERBB2: No Change |
%20II%20alpha&lib=Kinase&fbin=Discordant) Link |
%20II%20alpha&lib=Kinase&fbin=Discordant) Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
CAMK2A Aliases: CAMKA Entrez Gene: 815 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha mRNA: NM_015981 Library: Kinase |
%20II%20alpha&lib=Kinase) Link |
%20II%20alpha&lib=Kinase) Link |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
CAMK2D Aliases: CAMKD Entrez Gene: 817 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta mRNA: NM_001221 Library: Kinase Classification: Ser/Thr Kinase |
Final bin: No Phenotype SMARTpool bin: No change Avg area: 1.77 Avg Alamar: 0.94 Knockdown %: 60-70 |
%20II%20delta&lib=Kinase&fbin=No%20Phenotype) Link |
%20II%20delta&lib=Kinase&fbin=No%20Phenotype) Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
CAMK2D Aliases: CAMKD Entrez Gene: 817 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta mRNA: NM_001221 Library: Kinase |
%20II%20delta&lib=Kinase) Link |
%20II%20delta&lib=Kinase) Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00172636 | CAMK2D | CAMK2D PROTEIN. | 4 | 4 | 12 | 10 | 3.00 |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
CAMK2G Aliases: CAMK, CAMKG, CAMK-II Entrez Gene: 818 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma mRNA: NM_001222 Library: Kinase Classification: Ser/Thr Kinase |
Final bin: Discordant SMARTpool bin: Impaired Avg area: 2.43 Avg Alamar: 0.86 Knockdown %: 40-50 |
ERBB2: No Change |
%20II%20gamma&lib=Kinase&fbin=Discordant) Link |
%20II%20gamma&lib=Kinase&fbin=Discordant) Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
CAMK2G Aliases: CAMK, CAMKG, CAMK-II Entrez Gene: 818 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma mRNA: NM_001222 Library: Kinase |
%20II%20gamma&lib=Kinase) Link |
%20II%20gamma&lib=Kinase) Link |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
CaMKII Flybase Entrez CMCKB |
Calcium/calmodulin-dependent protein kinase II |
Mig: 202.4 Non-Mig: 189.7 |
1.06690 | 151686_s_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG18069 Entrez Gene: 43828 |
Calcium/calmodulin-dependent protein kinase II Aliases: calmodulin-dependent kinase; CaM kinase II; CaM kinase; calcium/ calmodulin-dependent protein kinase II; Ca[2+]/Calmodulin-dependent protein kinase II; calmodulin-dependent protein kinase II; Calmodulin kinase II; CaM-kinase-II; Ca[2+]/calmodulin-dependent protein kinase II |
14.500 1.157 |
1.17 | 1.056 |
Actin: 1.149 Area: 1.048 Ratio: 1.096 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
CAMK2B Aliases: CAM2, CAMK2, CAMKB Entrez Gene: 816 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II beta mRNA: NM_001220 Library: Kinase Classification: Ser/Thr Kinase |
Final bin: Impaired - HC SMARTpool bin: Impaired Avg area: 2.51 Avg Alamar: 0.85 Knockdown %: 80-90 |
ERBB2: No Change |
%20II%20beta&lib=Kinase&fbin=Impaired%20-%20HC) Link |
%20II%20beta&lib=Kinase&fbin=Impaired%20-%20HC) Link |
Impaired B Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
CAMK2B Aliases: CAM2, CAMK2, CAMKB Entrez Gene: 816 siRNA catalog |
calcium/calmodulin-dependent protein kinase (CaM kinase) II beta mRNA: NM_001220 Library: Kinase |
%20II%20beta&lib=Kinase) Link |
%20II%20beta&lib=Kinase) Link |
Consortium Related Products*
Biosensors » activity page
| Ortholog | Species | Name | Type | Source | Publications |
|---|---|---|---|---|---|
| CAMK2A | none | CaMKII | Intramolecular FRET between FPs - YFP-CaMKII-CFP |
-
|
Takao et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
The primary classification of CMKB families is the homeomorphic family, whose members are both homologous (evolved from a common ancestor) and homeomorphic (sharing full-length sequence similarity and a common domain architecture).