Cttn mouse: cortactin
Aliases: mammary tumor and squamous cell carcinoma associated (p80/85 src substrate), cortactin, Ems1, Cttn, 1110020L01Rik
Pfam Domain Structure
From Cttn (mouse) - phosphorylation sites added via direct mapping
Protein Overview
| Official Gene Name | Cttn (M. musculus) |
| RefSeq Protein Name | cortactin |
| UniProt Gene Symbol | SRC8_MOUSE |
| UniProt Name | Src substrate cortactin |
References
- Clark ES, Whigham AS, Yarbrough WG, Weaver AM. Cortactin is an essential regulator of matrix metalloproteinase secretion and extracellular matrix degradation in invadopodia. Cancer Res. 2007; 67:4227-35 (PubMed)
- Cosen-Binker LI, Kapus A. Cortactin: the gray eminence of the cytoskeleton. Physiology (Bethesda) 2006; 21:352-61 (PubMed)
- El Sayegh TY, Kapus A, McCulloch CA. Beyond the epithelium: cadherin function in fibrous connective tissues. FEBS Lett. 2007; 581:167-74 (PubMed)
- Lua BL, Low BC. Cortactin phosphorylation as a switch for actin cytoskeletal network and cell dynamics control. FEBS Lett. 2005; 579:577-85 (PubMed)
- Weaver AM. Cortactin in tumor invasiveness. Cancer Lett. 2008; 265:157-66 (PubMed)
- Weed SA, Parsons JT. Cortactin: coupling membrane dynamics to cortical actin assembly. Oncogene 2001; 20:6418-34 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| CTTN | none | phosphorylation site | Cortactin | Ectopic expression in HEK cells | 18 phospho-sites in 89.7% coverage of 543 aa | Parsons | Martin et al. |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
CTTN Aliases: EMS1 Entrez Gene: 2017 siRNA catalog |
cortactin mRNA: NM_005231 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: No Phenotype SMARTpool bin: No change Avg area: 1.41 Avg Alamar: 0.98 Knockdown %: not done |
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Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
CTTN Aliases: EMS1 Entrez Gene: 2017 siRNA catalog |
cortactin mRNA: NM_005231 Library: MAR |
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Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00062884 | CTTN | CTTN PROTEIN. | 19 | 12 | 44 | 22 | 2.32 |
| IPI00029601 | CTTN | SRC SUBSTRATE CORTACTIN. | 19 | 12 | 44 | 23 | 2.32 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00062884 | CTTN | CTTN PROTEIN. | 19 | 44 | 2.32 | cytoskeleton |
Proteomics» Table 3. Quantitation of phosphopeptides identified in Pseudopodium (PD) and Cell Body (CB) fractions
| IPI | Locus | Description | peptide | pdcb_p | pdcb_t | ratio | Mouse homology | Rat homology | Zebrafish homology | sd | Variable sites |
| IPI00029601 | CTTN | SRC SUBSTRATE CORTACTIN. | AKTQT*PPVS*PAPQPTEER | 1.89 | 2.32 | 0.82 | ++ | ++ | ++ | 0.02 | |
| IPI00029601 | CTTN | SRC SUBSTRATE CORTACTIN. | TQT*PPVS*PAPQPTEER | 1.47 | 2.32 | 0.63 | ++ | ++ | ++ | 0.18 | |
| IPI00029601 | CTTN | SRC SUBSTRATE CORTACTIN. | TQT*PPVS*PAPQPTEERLPSSPVYEDAASFK | 2.46 | 2.32 | 1.06 | ++ | ++ | 0.04 | ||
| IPI00062884 | CTTN | CTTN PROTEIN. | TQT*PPVSPAPQPTEERLPS*SPVYEDAASFK | 1.3 | 2.32 | 0.56 | ++ | ++ | 0.02 | T1S2 OR T1S3 |
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| NP_031829 | NM_007803 | Q60598 |
bold indicates the primary isoform
Pathways