DNM2 human: dynamin 2
Aliases: dynamin 2, dynamin II, DNM2, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII
Pfam Domain Structure
From DNM2 (human) - phosphorylation sites added via direct mapping
Protein Overview
| Official Gene Name | DNM2 (H. sapiens) |
| RefSeq Protein Name | dynamin 2 |
| UniProt Gene Symbol | DYN2_HUMAN |
| UniProt Name | Dynamin-2 |
| Entrez Gene Summary | Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq] |
References
- Ezratty EJ, Partridge MA, Gundersen GG. Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase. Nat. Cell Biol. 2005; 7:581-90 (PubMed)
- Orth JD, McNiven MA. Dynamin at the actin-membrane interface. Curr. Opin. Cell Biol. 2003; 15:31-9 (PubMed)
- Ramachandran R, Surka M, Chappie JS, Fowler DM, Foss TR, Song BD, Schmid SL. The dynamin middle domain is critical for tetramerization and higher-order self-assembly. EMBO J. 2007; 26:559-66 (PubMed)
- Shen H, Ferguson SM, Dephoure N, Park R, Yang Y, Volpicelli-Daley L, Gygi S, Schlessinger J, De Camilli P. Constitutive activated Cdc42-associated kinase (Ack) phosphorylation at arrested endocytic clathrin-coated pits of cells that lack dynamin. Mol. Biol. Cell 2011; 22:493-502 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| Dnm2 | none | phosphorylation site | Dynamin 2 | Ectopic expression in HEK cells | 8 phospho-sites in 89.4% coverage of 879 aa | Parsons |
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Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
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DNM2 Aliases: DYN2, DYNII, CMTDI1, CMTDIB Entrez Gene: 1785 siRNA catalog |
dynamin 2 mRNA: NM_004945 Library: MAR Classification: GTPase regulator Focal Adhesion Related: Yes |
Final bin: No Phenotype SMARTpool bin: No change Avg area: 1.87 Avg Alamar: 0.80 Knockdown %: not done |
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Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
DNM2 Aliases: DYN2, DYNII, CMTDI1, CMTDIB Entrez Gene: 1785 siRNA catalog |
dynamin 2 mRNA: NM_004945 Library: MAR |
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Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00033022 | DNM2 | SPLICE ISOFORM 1 OF DYNAMIN 2. | 36 | 17 | 52 | 24 | 1.44 |
| IPI00215974 | DNM2 | QUEUINE TRNA-RIBOSYLTRANSFERASE 1 (TRNA-GUANINE TRANSGLYCOSYLASE) . | 4 | 2 | 4 | 2 | 1.00 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00033022 | DNM2 | SPLICE ISOFORM 1 OF DYNAMIN 2. | 36 | 52 | 1.44 | cytoskeleton |
| IPI00033022 | DNM2 | SPLICE ISOFORM 1 OF DYNAMIN 2. | 36 | 52 | 1.44 | ribosome |
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| isoform 1 | NP_001005360 | NM_001005360 | P50570-1 |
| isoform 2 | NP_001005361 | NM_001005361 | P50570 |
| isoform 4 | NP_001005362 | NM_001005362 | P50570 |
| isoform 3 | NP_004936 | NM_004945 | P50570-2 |
| isoform 5 | NP_001177645 | NM_001190716 | P50570 |
bold indicates the primary isoform
Pathways