FLNA human: filamin A, alpha
Aliases: filamin 1, filamin A, alpha, actin-binding protein-280, FLN, FLNA, XX-FW83128A1.1, DKFZp434P031, OPD, FMD, MNS, ABPX, FLN1, NHBP, OPD1, OPD2, ABP-280
Pfam Domain Structure
From FLNA (human) - phosphorylation sites added via direct mapping
Protein Overview
| Official Gene Name | FLNA (H. sapiens) |
| RefSeq Protein Name | filamin A, alpha |
| UniProt Gene Symbol | FLNA_HUMAN |
| UniProt Name | Filamin-A |
| Entrez Gene Summary | The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene. |
References
- Feng Y, Walsh CA. The many faces of filamin: a versatile molecular scaffold for cell motility and signalling. Nat. Cell Biol. 2004; 6:1034-8 (PubMed)
- Gressens P. Pathogenesis of migration disorders. Curr. Opin. Neurol. 2006; 19:135-40 (PubMed)
- Guerrini R. Genetic malformations of the cerebral cortex and epilepsy. Epilepsia 2005; 46 Suppl 1:32-7 (PubMed)
- LoTurco JJ, Bai J. The multipolar stage and disruptions in neuronal migration. Trends Neurosci. 2006; 29:407-13 (PubMed)
- Lu J, Sheen V. Periventricular heterotopia. Epilepsy Behav 2005; 7:143-9 (PubMed)
- Nakamura F, Stossel TP, Hartwig JH. The filamins: Organizers of cell structure and function. Cell Adh Migr 2011; 5: (PubMed)
- Narayanan BA. Chemopreventive agents alters global gene expression pattern: predicting their mode of action and targets. Curr Cancer Drug Targets 2006; 6:711-27 (PubMed)
- Popowicz GM, Schleicher M, Noegel AA, Holak TA. Filamins: promiscuous organizers of the cytoskeleton. Trends Biochem. Sci. 2006; 31:411-9 (PubMed)
- Robertson SP. Filamin A: phenotypic diversity. Curr. Opin. Genet. Dev. 2005; 15:301-7 (PubMed)
- Sato M, Nagano T. Involvement of filamin A and filamin A-interacting protein (FILIP) in controlling the start and cell shape of radially migrating cortical neurons. Anat Sci Int 2005; 80:19-29 (PubMed)
- Sheetz MP, Sable JE, Döbereiner HG. Continuous membrane-cytoskeleton adhesion requires continuous accommodation to lipid and cytoskeleton dynamics. Annu Rev Biophys Biomol Struct 2006; 35:417-34 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| FLNA | none | phosphorylation site | Filamin-a | Endogenous from human platelet | 11 phospho-sites in 82.5% coverage of 2630 aa | Ginsberg |
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|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
FLNA Aliases: FLN, FMD, MNS, OPD, ABPX, FLN1, NHBP, OPD1, OPD2, ABP-280, filamin A Entrez Gene: 2316 siRNA catalog |
filamin A, alpha (actin binding protein 280) mRNA: NM_001456 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: Low Alamar Avg area: 2.90 Avg Alamar: 0.75 Knockdown %: not done |
ERBB2: Impaired BCL2: Low Alamar |
&lib=MAR&fbin=Discordant) Link |
&lib=MAR&fbin=Discordant) Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
FLNA Aliases: FLN, FMD, MNS, OPD, ABPX, FLN1, NHBP, OPD1, OPD2, ABP-280, filamin A Entrez Gene: 2316 siRNA catalog |
filamin A, alpha (actin binding protein 280) mRNA: NM_001456 Library: MAR |
&lib=MAR) Link |
&lib=MAR) Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00333541 | FLNA | FILAMIN A. | 445 | 116 | 821 | 145 | 1.84 |
| IPI00302592 | FLNA | FLJ00343 PROTEIN (FRAGMENT). | 438 | 113 | 801 | 141 | 1.83 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00333541 | FLNA | FILAMIN A. | 445 | 821 | 1.84 | Golgi |
| IPI00333541 | FLNA | FILAMIN A. | 445 | 821 | 1.84 | cytoskeleton |
Discovery» Drosophila Migration Genes
| Gene | Name | Function |
Cell Type |
Phenotypes |
Reagents/ Alleles |
Reference |
|
cher Entrez: CG3937 Flybase: FBgn0014141 |
cheerio | actin binding, Filamin | border cells |
Loss of function: incomplete border cell migration Autonomous: autonomous Gain of function: not reported Comments: can be rescued with actin-binding domain and 6 repeats |
cherEP3175, cherEPSdelta5, cherSko, cher1-6Hsp70 | Sokol 2003; Li 1999 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
cher Flybase Entrez CMCKB |
cheerio |
Mig: 550.2 Non-Mig: 658.4 |
0.83563 | 141641_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG3937 Entrez Gene: 42066 |
cheerio Aliases: filamin; Filamin; filamin-240; Filamin-A; Filamin-1; filamin B; shi kong |
19.938 1.475 |
1.201 | 1.077 |
Actin: 1.039 Area: 0.899 Ratio: 1.156 |
|
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| isoform 1 | NP_001447 | NM_001456 | P21333 |
| isoform 2 | NP_001104026 | NM_001110556 | P21333 |
bold indicates the primary isoform
Pathways