GIT1 human: G protein-coupled receptor kinase interacting ArfGAP 1
Aliases: G protein-coupled receptor kinase-interactor 1, GIT1
Pfam Domain Structure
From GIT1 (human) - phosphorylation sites added via direct mapping
Protein Overview
| Official Gene Name | GIT1 (H. sapiens) |
| RefSeq Protein Name | G protein-coupled receptor kinase interacting ArfGAP 1 |
| UniProt Gene Symbol | GIT1_HUMAN |
| UniProt Name | ARF GTPase-activating protein GIT1 |
References
- Frank SR, Hansen SH. The PIX-GIT complex: a G protein signaling cassette in control of cell shape. Semin. Cell Dev. Biol. 2008; 19:234-44 (PubMed)
- Hoefen RJ, Berk BC. The multifunctional GIT family of proteins. J. Cell. Sci. 2006; 119:1469-75 (PubMed)
- Premont RT, Perry SJ, Schmalzigaug R, Roseman JT, Xing Y, Claing A. The GIT/PIX complex: an oligomeric assembly of GIT family ARF GTPase-activating proteins and PIX family Rac1/Cdc42 guanine nucleotide exchange factors. Cell. Signal. 2004; 16:1001-11 (PubMed)
- Schmalzigaug R, Garron ML, Roseman JT, Xing Y, Davidson CE, Arold ST, Premont RT. GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin. Cell. Signal. 2007; 19:1733-44 (PubMed)
- Webb DJ, Mayhew MW, Kovalenko M, Schroeder MJ, Jeffery ED, Whitmore L, Shabanowitz J, Hunt DF, Horwitz AF. Identification of phosphorylation sites in GIT1. J. Cell. Sci. 2006; 119:2847-50 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| GIT1 | none | binding partner | GIT1 | Ectopic expression in HEK cells | 7 proteins exclusively co-purified with Git1 | Horwitz | Mayhew et al., Webb et al. |
| GIT1 | none | phosphorylation site | GIT1 | Ectopic expression in HEK cells | 28 phospho-sites in 89.9% coverage of 773 aa | Horwitz | Webb et al., Webb et al. |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
CG16728 Flybase Entrez CMCKB |
CG16728 gene product from transcript CG16728-RA |
Mig: 176.8 Non-Mig: 143.5 |
1.23210 | 154524_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG16728 Entrez Gene: 36122 |
9.813 1.139 |
0.795 | 0.906 |
Actin: 0.988 Area: 1.081 Ratio: 0.914 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
GIT1 Entrez Gene: 28964 siRNA catalog |
G protein-coupled receptor kinase interactor 1 mRNA: NM_014030 Library: MAR Classification: GTPase regulator Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: Impaired Avg area: 2.47 Avg Alamar: 0.89 Knockdown %: not done |
ERBB2: No Change |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
GIT1 Entrez Gene: 28964 siRNA catalog |
G protein-coupled receptor kinase interactor 1 mRNA: NM_014030 Library: MAR |
 Link |
 Link |
Consortium Related Products*
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| GIT1 | human | GIT1 - soluble expression | GIT1-baculovirus | 1-770 | GIT1 -full length | available | Liddington |
| GIT1 | human | GIT1 - soluble expression | pGEX4T3-GIT1c | 624-770 | GIT1 -paxillin binding domain | available | Liddington |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| isoform 2 | NP_054749 | NM_014030 | Q9Y2X7-1 |
| isoform 1 | NP_001078923 | NM_001085454 | Q9Y2X7 |
bold indicates the primary isoform
Pathways