PIK3CA human: phosphoinositide-3-kinase, catalytic, alpha polypeptide
Aliases: PtdIns-3-kinase p110, phosphoinositide-3-kinase, catalytic, alpha polypeptide, PI3-kinase p110 subunit alpha, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform, PI3K, PIK3CA, MGC142161, MGC142163, p110-alpha
Pfam Domain Structure
From PIK3CA (human)
Protein Overview
| Official Gene Name | PIK3CA (H. sapiens) |
| RefSeq Protein Name | phosphoinositide-3-kinase, catalytic, alpha polypeptide |
| UniProt Gene Symbol | PK3CA_HUMAN |
| UniProt Name | Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform |
| Entrez Gene Summary | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq] |
References
- Bader AG, Kang S, Zhao L, Vogt PK. Oncogenic PI3K deregulates transcription and translation. Nat. Rev. Cancer 2005; 5:921-9 (PubMed)
- Cain RJ, Ridley AJ. Phosphoinositide 3-kinases in cell migration. Biol. Cell 2009; 101:13-29 (PubMed)
- Carnero A, Blanco-Aparicio C, Renner O, Link W, Leal JF. The PTEN/PI3K/AKT signalling pathway in cancer, therapeutic implications. Curr Cancer Drug Targets 2008; 8:187-98 (PubMed)
- Funamoto S, Meili R, Lee S, Parry L, Firtel RA. Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis. Cell 2002; 109:611-23 (PubMed)
- Hoeller O, Kay RR. Chemotaxis in the absence of PIP3 gradients. Curr. Biol. 2007; 17:813-7 (PubMed)
- Houthoofd K, Fidalgo MA, Hoogewijs D, Braeckman BP, Lenaerts I, Brys K, Matthijssens F, De Vreese A, Van Eygen S, Muñoz MJ, Vanfleteren JR. Metabolism, physiology and stress defense in three aging Ins/IGF-1 mutants of the nematode Caenorhabditis elegans. Aging Cell 2005; 4:87-95 (PubMed)
- Kim JS, Lee C, Bonifant CL, Ressom H, Waldman T. Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA. Mol. Cell. Biol. 2007; 27:662-77 (PubMed)
- Kölsch V, Charest PG, Firtel RA. The regulation of cell motility and chemotaxis by phospholipid signaling. J. Cell. Sci. 2008; 121:551-9 (PubMed)
- Kumar A, Carrera AC. New functions for PI3K in the control of cell division. Cell Cycle 2007; 6:1696-8 (PubMed)
- Liu Z, Roberts TM. Human tumor mutants in the p110alpha subunit of PI3K. Cell Cycle 2006; 5:675-7 (PubMed)
- Weiger MC, Ahmed S, Welf ES, Haugh JM. Directional persistence of cell migration coincides with stability of asymmetric intracellular signaling. Biophys. J. 2010; 98:67-75 (PubMed)
- Yuan TL, Choi HS, Matsui A, Benes C, Lifshits E, Luo J, Frangioni JV, Cantley LC. Class 1A PI3K regulates vessel integrity during development and tumorigenesis. Proc. Natl. Acad. Sci. U.S.A. 2008; 105:9739-44 (PubMed)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
PIK3CA Aliases: PI3K, p110-alpha Entrez Gene: 5290 siRNA catalog |
phosphoinositide-3-kinase, catalytic, alpha polypeptide mRNA: NM_006218 Library: Kinase Classification: Lipid Kinase Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: Low Alamar Avg area: 1.82 Avg Alamar: 0.72 Knockdown %: 90-100 |
ERBB2: Impaired BCL2: Low Alamar |
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Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
PIK3CA Aliases: PI3K, p110-alpha Entrez Gene: 5290 siRNA catalog |
phosphoinositide-3-kinase, catalytic, alpha polypeptide mRNA: NM_006218 Library: Kinase |
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Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00031386 | PIK3CA | PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 3-KINASE CATALYTIC SUBUNIT, ALPHA ISOFORM. | 0 | 0 | 1 | 1 | PDU |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00031386 | PIK3CA | PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 3-KINASE CATALYTIC SUBUNIT, ALPHA ISOFORM. | 0 | 1 | PDU | cytosol |
Consortium Related Products*
Biosensors » activity page
| Ortholog | Species | Name | Type | Source | Publications |
|---|---|---|---|---|---|
| PIK3CA | none | PI3P | Probe redistribution - GFP-RING FYVE domains |
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|
Burd and Emr |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| isoform 2 | NP_006209 | NM_006218 | P42336 |
bold indicates the primary isoform
Pathways