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CAPN2 human: calpain 2, (m/II) large subunit

Aliases: Calcium-activated neutral proteinase 2, calpain, large polypeptide L2, calpain 2, large subunit, M-calpain, calpain M-type, millimolar-calpain, calpain 2, large [catalytic] subunit, calcium-activated neutral proteinase, CAPN2, FLJ39928, CANP 2, mCANP, CANP2, CANPL2, CANPml

Pfam Domain Structure

From CAPN2 (human) - phosphorylation sites added via direct mapping

Protein Overview
Official Gene Name CAPN2 (H. sapiens)
RefSeq Protein Name calpain 2, (m/II) large subunit
UniProt Gene Symbol CAN2_HUMAN
UniProt Name Calpain-2 catalytic subunit
Enzyme Commission 3.4.22.53
Entrez Gene Summary The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
References
  1. Carragher NO, Walker SM, Scott Carragher LA, Harris F, Sawyer TK, Brunton VG, Ozanne BW, Frame MC. Calpain 2 and Src dependence distinguishes mesenchymal and amoeboid modes of tumour cell invasion: a link to integrin function. Oncogene 2006; 25:5726-40 (PubMed)
  2. Chan KT, Bennin DA, Huttenlocher A. Regulation of adhesion dynamics by calpain-mediated proteolysis of focal adhesion kinase (FAK). J. Biol. Chem. 2010; 285:11418-26 (PubMed)
  3. Fernández-Montalván A, Assfalg-Machleidt I, Pfeiler D, Fritz H, Jochum M, Machleidt W. Mu-calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca2+-activated conformation. Biol. Chem. 2006; 387:617-27 (PubMed)
  4. Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6:977-83 (PubMed)
  5. Goll DE, Thompson VF, Li H, Wei W, Cong J. The calpain system. Physiol. Rev. 2003; 83:731-801 (PubMed)
  6. Huttenlocher A, Palecek SP, Lu Q, Zhang W, Mellgren RL, Lauffenburger DA, Ginsberg MH, Horwitz AF. Regulation of cell migration by the calcium-dependent protease calpain. J. Biol. Chem. 1997; 272:32719-22 (PubMed)
  7. Su LT, Agapito MA, Li M, Simonson WT, Huttenlocher A, Habas R, Yue L, Runnels LW. TRPM7 regulates cell adhesion by controlling the calcium-dependent protease calpain. J. Biol. Chem. 2006; 281:11260-70 (PubMed)
  8. Wu M, Yu Z, Fan J, Caron A, Whiteway M, Shen SH. Functional dissection of human protease mu-calpain in cell migration using RNAi. FEBS Lett. 2006; 580:3246-56 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
CAPN2 none binding partner Calpain 2 Ectopic expression in HEK cells Proteins co-purifying with expressed FLAG-tagged calpain Horwitz
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CAPN2 none phosphorylation site Calpain 2 Ectopic expression in HEK cells 4 phospho-sites in 88.1% coverage of 708 aa Parsons
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Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
CAPN2
Aliases: mCANP, CANPL2, CANPml
Entrez Gene: 824
siRNA catalog 		calpain 2, (m/II) large subunit
mRNA: NM_001748
Library: MAR
Classification: protease
 Final bin: Discordant
SMARTpool bin: Low Alamar
Avg area: 2.94
Avg Alamar: 0.68
Knockdown %: not done
 ERBB2: Impaired
BCL2: Low Alamar 		CAPN2
Link 		CAPN2
Link 		 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
CAPN2
Aliases: mCANP, CANPL2, CANPml
Entrez Gene: 824
siRNA catalog 		calpain 2, (m/II) large subunit
mRNA: NM_001748
Library: MAR
 CAPN2
Link 		CAPN2
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00289758 CAPN2 CALPAIN 2, LARGE [CATALYTIC] SUBUNIT PRECURSOR. 41 15 41 20 1.00
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
CalpB
Flybase
Entrez
CMCKB
 Calpain-B Mig: 307.8
Non-Mig: 448.9
 0.68568 151648_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization 		Stellate11
NumCorners 		Stellate22
Binsum6 		Non-Spreading3
CG8107
Entrez Gene: 39165
 Calpain-B
Aliases: Calpain B; calpain B 		11.875
1.558 		1.028 1.024 Actin: 1.097
Area: 0.846
Ratio: 1.297
Consortium Related Products*
Mice » activity page
Ortholog Species Name Method Phenotype Status Jax Availability Source Publications
CAPN2 mouse Calpain II (m-calpain) (Capn2)
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 None, though elimination of the Capn4 gene (see below) destroys the CalpainII complex. completed
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 Arthur et al.

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

Isoforms
Isoform Name RefSeq Protein RefSeq mRNA Swissprot ID
NP_001739 NM_001748 P17655
NP_001139540 NM_001146068 P17655

bold indicates the primary isoform