NCK2 human: NCK adaptor protein 2
Aliases: NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta, noncatalytic region of tyrosine kinase, beta, GRB4, NCK2, NCKbeta
Pfam Domain Structure
From NCK2 (human)
Protein Overview
| Official Gene Name | NCK2 (H. sapiens) |
| RefSeq Protein Name | NCK adaptor protein 2 |
| UniProt Gene Symbol | NCK2_HUMAN |
| UniProt Name | Cytoplasmic protein NCK2 |
| Entrez Gene Summary | This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
References
- Baumgartner M, Sillman AL, Blackwood EM, Srivastava J, Madson N, Schilling JW, Wright JH, Barber DL. The Nck-interacting kinase phosphorylates ERM proteins for formation of lamellipodium by growth factors. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:13391-6 (PubMed)
- Blasutig IM, New LA, Thanabalasuriar A, Dayarathna TK, Goudreault M, Quaggin SE, Li SS, Gruenheid S, Jones N, Pawson T. Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization. Mol. Cell. Biol. 2008; 28:2035-46 (PubMed)
- Buday L, Wunderlich L, Tamás P. The Nck family of adapter proteins: regulators of actin cytoskeleton. Cell. Signal. 2002; 14:723-31 (PubMed)
- Li W, Fan J, Woodley DT. Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 2001; 20:6403-17 (PubMed)
- Rivera GM, Antoku S, Gelkop S, Shin NY, Hanks SK, Pawson T, Mayer BJ. Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:9536-41 (PubMed)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
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NCK2 Aliases: GRB4, NCKbeta Entrez Gene: 8440 siRNA catalog |
NCK adaptor protein 2 mRNA: NM_003581 Library: MAR Classification: adaptor Focal Adhesion Related: Yes |
Final bin: Low Alamar - MC SMARTpool bin: Low Alamar Avg area: 3.67 Avg Alamar: 0.59 Knockdown %: not done |
ERBB2: No Change BCL2: Low Alamar |
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Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
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NCK2 Aliases: GRB4, NCKbeta Entrez Gene: 8440 siRNA catalog |
NCK adaptor protein 2 mRNA: NM_003581 Library: MAR |
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Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| NCK2 | mouse | Nck1 / Nck2 - embryonic fibroblasts | - |
Defects in cell motility and organization of the lamellipodial actin network. | completed |
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Bladt et al. |
| NCK2 | mouse | Nck1 / Nck2 | - |
Nck genes have broad and overlapping expression pattern and mice deficient in either Nck1 or Nck2 are viable, whereas inactivation of both resulted in profound defects in mesoderm-derived notochord and embryonic lethality at day 9.5. | completed |
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Bladt et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| isoform b | NP_001004722 | NM_001004722 | O43639 |
| isoform a | NP_001004720 | NM_001004720 | O43639 |
| isoform a | NP_003572 | NM_003581 | O43639 |
bold indicates the primary isoform
Pathways