ACTN1 human: actinin, alpha 1
Aliases: actinin, alpha 1, alpha-actinin 1, actinin 1 smooth muscle, F-actin cross-linking protein, ACTN1, FLJ40884, FLJ54432
Pfam Domain Structure
From ACTN1 (human) - phosphorylation sites added via direct mapping
Protein Overview
| Official Gene Name | ACTN1 (H. sapiens) |
| RefSeq Protein Name | actinin, alpha 1 |
| UniProt Gene Symbol | ACTN1_HUMAN |
| UniProt Name | Alpha-actinin-1 |
| Entrez Gene Summary | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
References
- Cabello N, Remelli R, Canela L, Soriguera A, Mallol J, Canela EI, Robbins MJ, Lluis C, Franco R, McIlhinney RA, Ciruela F. Actin-binding protein alpha-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor. J. Biol. Chem. 2007; 282:12143-53 (PubMed)
- Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 2008; 10:1039-50 (PubMed)
- Courson DS, Rock RS. Actin cross-link assembly and disassembly mechanics for alpha-Actinin and fascin. J. Biol. Chem. 2010; 285:26350-7 (PubMed)
- Izaguirre G, Aguirre L, Hu YP, Lee HY, Schlaepfer DD, Aneskievich BJ, Haimovich B. The cytoskeletal/non-muscle isoform of alpha-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase. J. Biol. Chem. 2001; 276:28676-85 (PubMed)
- Lazarides E, Burridge K. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells. Cell 1975; 6:289-98 (PubMed)
- Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
- Otey CA, Pavalko FM, Burridge K. An interaction between alpha-actinin and the beta 1 integrin subunit in vitro. J. Cell Biol. 1990; 111:721-9 (PubMed)
- Wahlström G, Norokorpi HL, Heino TI. Drosophila alpha-actinin in ovarian follicle cells is regulated by EGFR and Dpp signalling and required for cytoskeletal remodelling. Mech. Dev. 2006; 123:801-18 (PubMed)
- Zhang W, Gunst SJ. Dynamic association between alpha-actinin and beta-integrin regulates contraction of canine tracheal smooth muscle. J. Physiol. (Lond.) 2006; 572:659-76 (PubMed)
- Zhang Z, Lin SY, Neel BG, Haimovich B. Phosphorylated alpha-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase x Src complex and promote cell migration. J. Biol. Chem. 2006; 281:1746-54 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| ACTN1 | none | structure | Alpha-actinin cross-links | 2D crystallization on lipid monolayers | completed | Taylor | Hampton et al. |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
ACTN1 Entrez Gene: 87 siRNA catalog |
actinin, alpha 1 mRNA: NM_001102 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: No change Avg area: 1.79 Avg Alamar: 0.96 Knockdown %: not done |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
ACTN1 Entrez Gene: 87 siRNA catalog |
actinin, alpha 1 mRNA: NM_001102 Library: MAR |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00013508 | ACTN1 | ALPHA-ACTININ 1. | 305 | 61 | 549 | 77 | 1.80 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00013508 | ACTN1 | ALPHA-ACTININ 1. | 305 | 549 | 1.8 | cytoskeleton |
Discovery» Genes encoding cytoskeleton-associated proteins enriched in migratory cells
| Gene | Description | Nearest mammalian (Human) homolog | Fold Change |
|
Actn GeneID:31166 Entrez Flybase |
alpha-actinin Proposed Function: F-actin crosslinking Links talin and F-actin Conserved Protein Domains: CH, EF hand, Spectrin repeats |
ACTN1 GeneID: 87 CMKB Orthologs |
1.38
|
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
Actn Flybase Entrez CMCKB |
alpha actinin |
Mig: 1096.2 Non-Mig: 739.5 |
1.48240 | 154816_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG4376 Entrez Gene: 31166 |
alpha actinin Aliases: Alpha-actinin; alpha-actinin; flightless A; alpha-Actinin; lethal(1)2cb; a-actinin; flightless-A |
13.188 1.081 |
1.012 | 0.973 |
Actin: 0.886 Area: 1.117 Ratio: 0.793 |
|
Consortium Related Products*
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| ACTN1 | human | Alpha Actinin - soluble | pET15b-aActinin-SR | 269-444 | Alpha actinin - spectrin repeats | - |
Liddington |
| ACTN1 | human | Alpha Actinin - soluble | pET15b-aActinin | 1-892 | Alpha actinin - full length | available | Liddington |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| isoform b | NP_001093 | NM_001102 | P12814 |
| isoform a | NP_001123476 | NM_001130004 | P12814 |
| isoform c | NP_001123477 | NM_001130005 | P12814 |
bold indicates the primary isoform
Pathways