PTK2 Orthologs
Aliases: focal adhesion kinase 1, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, FADK 1, PTK2, FADK, FAK1, pp125FAK
Processes & Diseases
Processes involved: adhesion
Pfam Domain Structure
From PTK2 (chicken) - phosphorylation sites added via direct mapping
Members of the PTK2 ortholog set
| Human | Mouse | Rat | Chicken | Fly | Worm | |
| CMKB | PTK2 | Ptk2 | Ptk2 | PTK2 | Fak56D | kin-32 |
| Entrez Gene | 5747 | 14083 | 25614 | 396416 | 37233 | 172135 |
| Uniprot | FAK1_HUMAN | FAK1_MOUSE | FAK1_RAT | FAK1_CHICK | - | - |
| OMIM | 600758 | - | - | - | - | - |
| Reagents | 2 available | 4 available | - | - | - | - |
| Results | 4 available | 1 available | - | - | 1 available | - |
References
- Cohen LA, Guan JL. Mechanisms of focal adhesion kinase regulation. Curr Cancer Drug Targets 2005; 5:629-43 (PubMed)
- Cox BD, Natarajan M, Stettner MR, Gladson CL. New concepts regarding focal adhesion kinase promotion of cell migration and proliferation. J. Cell. Biochem. 2006; 99:35-52 (PubMed)
- Lim ST, Mikolon D, Stupack DG, Schlaepfer DD. FERM control of FAK function: implications for cancer therapy. Cell Cycle 2008; 7:2306-14 (PubMed)
- Mitra SK, Schlaepfer DD. Integrin-regulated FAK-Src signaling in normal and cancer cells. Curr. Opin. Cell Biol. 2006; 18:516-23 (PubMed)
- Mon NN, Ito S, Senga T, Hamaguchi M. FAK signaling in neoplastic disorders: a linkage between inflammation and cancer. Ann. N. Y. Acad. Sci. 2006; 1086:199-212 (PubMed)
- Parsons JT. Focal adhesion kinase: the first ten years. J. Cell. Sci. 2003; 116:1409-16 (PubMed)
- Schaller MD. FAK and paxillin: regulators of N-cadherin adhesion and inhibitors of cell migration?. J. Cell Biol. 2004; 166:157-9 (PubMed)
- Tomar A, Schlaepfer DD. Focal adhesion kinase: switching between GAPs and GEFs in the regulation of cell motility. Curr. Opin. Cell Biol. 2009; 21:676-83 (PubMed)
- Webb DJ, Donais K, Whitmore LA, Thomas SM, Turner CE, Parsons JT, Horwitz AF. FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly. Nat. Cell Biol. 2004; 6:154-61 (PubMed)
- Wozniak MA, Modzelewska K, Kwong L, Keely PJ. Focal adhesion regulation of cell behavior. Biochim. Biophys. Acta 2004; 1692:103-19 (PubMed)
- Xie Z, Tsai LH. Cdk5 phosphorylation of FAK regulates centrosome-associated miocrotubules and neuronal migration. Cell Cycle 2004; 3:108-10 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| PTK2 | none | phosphorylation site | FAK | Ectopic expression in HEK cells | 18 phospho-sites in 80.2% coverage of 1045 aa | Parsons | Grigera et al. |
| PTK2 | none | structure | FAT domain of Focal Adhesion Kinase | X-Ray crystallography, NMR | data available | Liddington | Hayashi et al. |
Discovery» MTLn3 invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Ptk2 Entrez Gene: 14083 |
mRNA: AW536852 UniGene: Mm.254494 |
PTK2 protein tyrosine kinase 2 | 2.2 |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG10023 Entrez Gene: 37233 |
Focal Adhesion Kinase |
7.688 1.202 |
1.041 | 1.061 |
Actin: 0.978 Area: 0.91 Ratio: 1.075 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
PTK2 Aliases: FAK, FADK, FAK1, pp125FAK Entrez Gene: 5747 siRNA catalog |
PTK2 protein tyrosine kinase 2 mRNA: NM_005607 Library: Kinase Classification: Ser/Thr Kinase Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: No change Avg area: 1.28 Avg Alamar: 0.83 Knockdown %: 70-80 |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
PTK2 Aliases: FAK, FADK, FAK1, pp125FAK Entrez Gene: 5747 siRNA catalog |
PTK2 protein tyrosine kinase 2 mRNA: NM_005607 Library: Kinase |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00012885 | PTK2 | SPLICE ISOFORM 1 OF FOCAL ADHESION KINASE 1. | 4 | 4 | 14 | 8 | 3.50 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00012885 | PTK2 | SPLICE ISOFORM 1 OF FOCAL ADHESION KINASE 1. | 4 | 14 | 3.5 | cytoskeleton |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| PTK2 | mouse | FAK / Cas - double knockout in embryonic fibroblasts | - |
Defects in ephrinA1-induced cell spreading. | completed |
-
|
-
|
Carter et al. |
| PTK2 | mouse | Fak | - |
Death at E8.5 with impaired blood vessel development. | completed |
-
|
-
|
Ilic et al. |
| PTK2 | mouse | FAK - embryonic fibroblasts | - |
Reduced migration rate and an increase in the number and size of peripherally localized adhesions. | completed |
-
|
-
|
Ilić et al. |
| PTK2 | mouse | FAK | - |
Elevated levels of Pyk2. | completed |
-
|
-
|
Sieg et al. |
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| PTK2 | human | FAK - soluble expression | FAK-baculovirus | 1-1052 | FAK-full length | - |
Liddington |
| PTK2 | human | FAK | pT7-7-FAK | 1-1052 | FAK-full length | available | Liddington |
Biosensors » activity page
| Ortholog | Species | Name | Type | Source | Publications |
|---|---|---|---|---|---|
| PTK2 | none | FAK | Intermolecular FRET between FPs - CFP-FAK; dSH2 (Src)-YFP |
-
|
Cai et al. |
| PTK2 | none | FAK | Intramolecular FRET between FPs - Insertion of a FRET pair in FAK |
-
|
Cai et al. |
| PTK2 | none | FAK | FRET - single chain | Hahn | Cai et al. |
Imaging Probes » activity page
| Ortholog | Species | Name | Type | Status | Source | Publications |
|---|---|---|---|---|---|---|
| PTK2 | none | Photoactivation | Fak/PTK2 - (caged phosphoTyr (Y*) - cPYFAK, probe sequence; VSETDDY*AEIIDEEDT-NH2) | completed | Imperiali, Jacobson | Humphrey et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Pathways