ACTB Orthologs

Aliases: beta cytoskeletal actin, beta actin, actin, cytoplasmic 1, PS1TP5-binding protein 1, ACTB, PS1TP5BP1

Processes & Diseases

Processes involved: protrusion, polarity

Pfam Domain Structure

From ACTB (human)

Members of the ACTB ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB ACTB Actb Actb - Act57B act-2
Entrez Gene 60 11461 81822 - 37368 179534
Uniprot ACTB_HUMAN ACTB_MOUSE ACTB_RAT - ACT3_DROME ACT2_CAEEL
OMIM 102630 - - - - -
Reagents - - - - - -
Results 4 available 2 available - - 2 available -
References
  1. Aepfelbacher M, Zumbihl R, Heesemann J. Modulation of Rho GTPases and the actin cytoskeleton by YopT of Yersinia. Curr. Top. Microbiol. Immunol. 2005; 291:167-75 (PubMed)
  2. Akiyama T, Kawasaki Y. Wnt signalling and the actin cytoskeleton. Oncogene 2006; 25:7538-44 (PubMed)
  3. Alexandrova AY, Arnold K, Schaub S, Vasiliev JM, Meister JJ, Bershadsky AD, Verkhovsky AB. Comparative dynamics of retrograde actin flow and focal adhesions: formation of nascent adhesions triggers transition from fast to slow flow. PLoS ONE 2008; 3:e3234 (PubMed)
  4. Bannigan A, Baskin TI. Directional cell expansion--turning toward actin. Curr. Opin. Plant Biol. 2005; 8:619-24 (PubMed)
  5. Baum J, Papenfuss AT, Baum B, Speed TP, Cowman AF. Regulation of apicomplexan actin-based motility. Nat. Rev. Microbiol. 2006; 4:621-8 (PubMed)
  6. Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 2008; 10:1039-50 (PubMed)
  7. Condeelis J. How is actin polymerization nucleated in vivo?. Trends Cell Biol. 2001; 11:288-93 (PubMed)
  8. Cooper JA, Schafer DA. Control of actin assembly and disassembly at filament ends. Curr. Opin. Cell Biol. 2000; 12:97-103 (PubMed)
  9. Cramer LP, Siebert M, Mitchison TJ. Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: implications for the generation of motile force. J. Cell Biol. 1997; 136:1287-305 (PubMed)
  10. DeFea KA. Stop that cell! Beta-arrestin-dependent chemotaxis: a tale of localized actin assembly and receptor desensitization. Annu. Rev. Physiol. 2007; 69:535-60 (PubMed)
  11. Delon I, Brown NH. Integrins and the actin cytoskeleton. Curr. Opin. Cell Biol. 2007; 19:43-50 (PubMed)
  12. Disanza A, Steffen A, Hertzog M, Frittoli E, Rottner K, Scita G. Actin polymerization machinery: the finish line of signaling networks, the starting point of cellular movement. Cell. Mol. Life Sci. 2005; 62:955-70 (PubMed)
  13. Goley ED, Welch MD. The ARP2/3 complex: an actin nucleator comes of age. Nat. Rev. Mol. Cell Biol. 2006; 7:713-26 (PubMed)
  14. Hotulainen P, Lappalainen P. Stress fibers are generated by two distinct actin assembly mechanisms in motile cells. J. Cell Biol. 2006; 173:383-94 (PubMed)
  15. Ibarra N, Pollitt A, Insall RH. Regulation of actin assembly by SCAR/WAVE proteins. Biochem. Soc. Trans. 2005; 33:1243-6 (PubMed)
  16. Insall RH, Machesky LM. Actin dynamics at the leading edge: from simple machinery to complex networks. Dev. Cell 2009; 17:310-22 (PubMed)
  17. Keren K, Pincus Z, Allen GM, Barnhart EL, Marriott G, Mogilner A, Theriot JA. Mechanism of shape determination in motile cells. Nature 2008; 453:475-80 (PubMed)
  18. Kislauskis EH, Zhu X, Singer RH. beta-Actin messenger RNA localization and protein synthesis augment cell motility. J. Cell Biol. 1997; 136:1263-70 (PubMed)
  19. Korn ED, Carlier MF, Pantaloni D. Actin polymerization and ATP hydrolysis. Science 1987; 238:638-44 (PubMed)
  20. Larsson C. Protein kinase C and the regulation of the actin cytoskeleton. Cell. Signal. 2006; 18:276-84 (PubMed)
  21. Lee K, Gallop JL, Rambani K, Kirschner MW. Self-assembly of filopodia-like structures on supported lipid bilayers. Science 2010; 329:1341-5 (PubMed)
  22. Machesky LM, Hall A. Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization. J. Cell Biol. 1997; 138:913-26 (PubMed)
  23. Marston DJ, Goldstein B. Actin-based forces driving embryonic morphogenesis in Caenorhabditis elegans. Curr. Opin. Genet. Dev. 2006; 16:392-8 (PubMed)
  24. Mseka T, Coughlin M, Cramer LP. Graded actin filament polarity is the organization of oriented actomyosin II filament bundles required for fibroblast polarization. Cell Motil. Cytoskeleton 2009; 66:743-53 (PubMed)
  25. Nicholson-Dykstra S, Higgs HN, Harris ES. Actin dynamics: growth from dendritic branches. Curr. Biol. 2005; 15:R346-57 (PubMed)
  26. Oda T, Stegmann H, Schröder RR, Namba K, Maéda Y. Modeling of the F-actin structure. Adv. Exp. Med. Biol. 2007; 592:385-401 (PubMed)
  27. Pollard TD, Cooper JA. Actin, a central player in cell shape and movement. Science 2009; 326:1208-12 (PubMed)
  28. Ponti A, Machacek M, Gupton SL, Waterman-Storer CM, Danuser G. Two distinct actin networks drive the protrusion of migrating cells. Science 2004; 305:1782-6 (PubMed)
  29. Ridley AJ. Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol. 2006; 16:522-9 (PubMed)
  30. Rubenstein PA, Wen KK. Lights, camera, actin. IUBMB Life 2005; 57:683-7 (PubMed)
  31. Small JV, Resch GP. The comings and goings of actin: coupling protrusion and retraction in cell motility. Curr. Opin. Cell Biol. 2005; 17:517-23 (PubMed)
  32. Staiger CJ, Blanchoin L. Actin dynamics: old friends with new stories. Curr. Opin. Plant Biol. 2006; 9:554-62 (PubMed)
  33. Van Haastert PJ, Devreotes PN. Chemotaxis: signalling the way forward. Nat. Rev. Mol. Cell Biol. 2004; 5:626-34 (PubMed)
  34. Weis WI, Nelson WJ. Re-solving the cadherin-catenin-actin conundrum. J. Biol. Chem. 2006; 281:35593-7 (PubMed)
  35. Yamazaki D, Kurisu S, Takenawa T. Regulation of cancer cell motility through actin reorganization. Cancer Sci. 2005; 96:379-86 (PubMed)
  36. Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J. Cell Biol. 2007; 178:1207-21 (PubMed)
  37. Zigmond SH. Beginning and ending an actin filament: control at the barbed end. Curr. Top. Dev. Biol. 2004; 63:145-88 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
ACTB none structure F-actin-villin (with David DeRosier) Electron Tomography completed Taylor Hampton et al.
ACTB none structure Alpha-actinin2/F-actin rafts lipid monolayer, negative stain, single particle image processing completed Taylor
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Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
Act57B
Flybase
Entrez
CMCKB
Actin 57B Mig: 5130.0
Non-Mig: 2929.0
1.75150 153531_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG10067
Entrez Gene: 37368
Actin 57B
Aliases: actin; Actin; Actin57B; actin57B
7.400
1.157
1.634 1.418 Actin: 0.34
Area: 0.226
Ratio: 1.504
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
ACTB
Aliases: PS1TP5BP1
Entrez Gene: 60
siRNA catalog
actin, beta
mRNA: NM_001101
Library: MAR
Classification: cytoskeletal
Focal Adhesion Related: Yes
Final bin: Impaired - HC
SMARTpool bin: Impaired
Avg area: 4.06
Avg Alamar: 0.92
Knockdown %: not done
ERBB2: No Change
ACTB
Link
ACTB
Link
Impaired unique
ACTB
Link
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
ACTB
Aliases: PS1TP5BP1
Entrez Gene: 60
siRNA catalog
actin, beta
mRNA: NM_001101
Library: MAR
ACTB
Link
ACTB
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00021439 ACTB ACTIN, CYTOPLASMIC 1. 997 59 1875 79 1.88
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00021439 ACTB ACTIN, CYTOPLASMIC 1. 997 1875 1.88 nucleus
IPI00021439 ACTB ACTIN, CYTOPLASMIC 1. 997 1875 1.88 cytoskeleton
IPI00021439 ACTB ACTIN, CYTOPLASMIC 1. 997 1875 1.88 cytosol
Discovery» MTLn3 invasive data
Gene mRNA Description Expression Ratio
Actb
Entrez Gene: 11461
mRNA: C78835
UniGene: Mm.391967
Actin, beta, cytoplasmic 2.9
Discovery» PyMT invasive data
Gene mRNA Description Expression Ratio
Actb
Entrez Gene: 11461
mRNA: AW544311
UniGene: Mm.455830
Actin, beta, cytoplasmic 0.19
Actb
Entrez Gene: 11461
mRNA: AW544311
UniGene: Mm.455830
Actin, beta, cytoplasmic 0.18
Pathways

How many degrees of interaction?

Update
Legend: Legend for CMKB pathways