ACTB Orthologs
Aliases: beta cytoskeletal actin, beta actin, actin, cytoplasmic 1, PS1TP5-binding protein 1, ACTB, PS1TP5BP1
Processes & Diseases
Processes involved: protrusion, polarity
Pfam Domain Structure
From ACTB (human)
Members of the ACTB ortholog set
| Human | Mouse | Rat | Chicken | Fly | Worm | |
| CMKB | ACTB | Actb | Actb | - | Act57B | act-2 |
| Entrez Gene | 60 | 11461 | 81822 | - | 37368 | 179534 |
| Uniprot | ACTB_HUMAN | ACTB_MOUSE | ACTB_RAT | - | ACT3_DROME | ACT2_CAEEL |
| OMIM | 102630 | - | - | - | - | - |
| Reagents | - | - | - | - | - | - |
| Results | 4 available | 2 available | - | - | 2 available | - |
References
- Aepfelbacher M, Zumbihl R, Heesemann J. Modulation of Rho GTPases and the actin cytoskeleton by YopT of Yersinia. Curr. Top. Microbiol. Immunol. 2005; 291:167-75 (PubMed)
- Akiyama T, Kawasaki Y. Wnt signalling and the actin cytoskeleton. Oncogene 2006; 25:7538-44 (PubMed)
- Alexandrova AY, Arnold K, Schaub S, Vasiliev JM, Meister JJ, Bershadsky AD, Verkhovsky AB. Comparative dynamics of retrograde actin flow and focal adhesions: formation of nascent adhesions triggers transition from fast to slow flow. PLoS ONE 2008; 3:e3234 (PubMed)
- Bannigan A, Baskin TI. Directional cell expansion--turning toward actin. Curr. Opin. Plant Biol. 2005; 8:619-24 (PubMed)
- Baum J, Papenfuss AT, Baum B, Speed TP, Cowman AF. Regulation of apicomplexan actin-based motility. Nat. Rev. Microbiol. 2006; 4:621-8 (PubMed)
- Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 2008; 10:1039-50 (PubMed)
- Condeelis J. How is actin polymerization nucleated in vivo?. Trends Cell Biol. 2001; 11:288-93 (PubMed)
- Cooper JA, Schafer DA. Control of actin assembly and disassembly at filament ends. Curr. Opin. Cell Biol. 2000; 12:97-103 (PubMed)
- Cramer LP, Siebert M, Mitchison TJ. Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: implications for the generation of motile force. J. Cell Biol. 1997; 136:1287-305 (PubMed)
- DeFea KA. Stop that cell! Beta-arrestin-dependent chemotaxis: a tale of localized actin assembly and receptor desensitization. Annu. Rev. Physiol. 2007; 69:535-60 (PubMed)
- Delon I, Brown NH. Integrins and the actin cytoskeleton. Curr. Opin. Cell Biol. 2007; 19:43-50 (PubMed)
- Disanza A, Steffen A, Hertzog M, Frittoli E, Rottner K, Scita G. Actin polymerization machinery: the finish line of signaling networks, the starting point of cellular movement. Cell. Mol. Life Sci. 2005; 62:955-70 (PubMed)
- Goley ED, Welch MD. The ARP2/3 complex: an actin nucleator comes of age. Nat. Rev. Mol. Cell Biol. 2006; 7:713-26 (PubMed)
- Hotulainen P, Lappalainen P. Stress fibers are generated by two distinct actin assembly mechanisms in motile cells. J. Cell Biol. 2006; 173:383-94 (PubMed)
- Ibarra N, Pollitt A, Insall RH. Regulation of actin assembly by SCAR/WAVE proteins. Biochem. Soc. Trans. 2005; 33:1243-6 (PubMed)
- Insall RH, Machesky LM. Actin dynamics at the leading edge: from simple machinery to complex networks. Dev. Cell 2009; 17:310-22 (PubMed)
- Keren K, Pincus Z, Allen GM, Barnhart EL, Marriott G, Mogilner A, Theriot JA. Mechanism of shape determination in motile cells. Nature 2008; 453:475-80 (PubMed)
- Kislauskis EH, Zhu X, Singer RH. beta-Actin messenger RNA localization and protein synthesis augment cell motility. J. Cell Biol. 1997; 136:1263-70 (PubMed)
- Korn ED, Carlier MF, Pantaloni D. Actin polymerization and ATP hydrolysis. Science 1987; 238:638-44 (PubMed)
- Larsson C. Protein kinase C and the regulation of the actin cytoskeleton. Cell. Signal. 2006; 18:276-84 (PubMed)
- Lee K, Gallop JL, Rambani K, Kirschner MW. Self-assembly of filopodia-like structures on supported lipid bilayers. Science 2010; 329:1341-5 (PubMed)
- Machesky LM, Hall A. Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization. J. Cell Biol. 1997; 138:913-26 (PubMed)
- Marston DJ, Goldstein B. Actin-based forces driving embryonic morphogenesis in Caenorhabditis elegans. Curr. Opin. Genet. Dev. 2006; 16:392-8 (PubMed)
- Mseka T, Coughlin M, Cramer LP. Graded actin filament polarity is the organization of oriented actomyosin II filament bundles required for fibroblast polarization. Cell Motil. Cytoskeleton 2009; 66:743-53 (PubMed)
- Nicholson-Dykstra S, Higgs HN, Harris ES. Actin dynamics: growth from dendritic branches. Curr. Biol. 2005; 15:R346-57 (PubMed)
- Oda T, Stegmann H, Schröder RR, Namba K, Maéda Y. Modeling of the F-actin structure. Adv. Exp. Med. Biol. 2007; 592:385-401 (PubMed)
- Pollard TD, Cooper JA. Actin, a central player in cell shape and movement. Science 2009; 326:1208-12 (PubMed)
- Ponti A, Machacek M, Gupton SL, Waterman-Storer CM, Danuser G. Two distinct actin networks drive the protrusion of migrating cells. Science 2004; 305:1782-6 (PubMed)
- Ridley AJ. Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol. 2006; 16:522-9 (PubMed)
- Rubenstein PA, Wen KK. Lights, camera, actin. IUBMB Life 2005; 57:683-7 (PubMed)
- Small JV, Resch GP. The comings and goings of actin: coupling protrusion and retraction in cell motility. Curr. Opin. Cell Biol. 2005; 17:517-23 (PubMed)
- Staiger CJ, Blanchoin L. Actin dynamics: old friends with new stories. Curr. Opin. Plant Biol. 2006; 9:554-62 (PubMed)
- Van Haastert PJ, Devreotes PN. Chemotaxis: signalling the way forward. Nat. Rev. Mol. Cell Biol. 2004; 5:626-34 (PubMed)
- Weis WI, Nelson WJ. Re-solving the cadherin-catenin-actin conundrum. J. Biol. Chem. 2006; 281:35593-7 (PubMed)
- Yamazaki D, Kurisu S, Takenawa T. Regulation of cancer cell motility through actin reorganization. Cancer Sci. 2005; 96:379-86 (PubMed)
- Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J. Cell Biol. 2007; 178:1207-21 (PubMed)
- Zigmond SH. Beginning and ending an actin filament: control at the barbed end. Curr. Top. Dev. Biol. 2004; 63:145-88 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| ACTB | none | structure | F-actin-villin (with David DeRosier) | Electron Tomography | completed | Taylor | Hampton et al. |
| ACTB | none | structure | Alpha-actinin2/F-actin rafts | lipid monolayer, negative stain, single particle image processing | completed | Taylor |
-
|
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
Act57B Flybase Entrez CMCKB |
Actin 57B |
Mig: 5130.0 Non-Mig: 2929.0 |
1.75150 | 153531_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG10067 Entrez Gene: 37368 |
Actin 57B Aliases: actin; Actin; Actin57B; actin57B |
7.400 1.157 |
1.634 | 1.418 |
Actin: 0.34 Area: 0.226 Ratio: 1.504 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
ACTB Aliases: PS1TP5BP1 Entrez Gene: 60 siRNA catalog |
actin, beta mRNA: NM_001101 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: Impaired - HC SMARTpool bin: Impaired Avg area: 4.06 Avg Alamar: 0.92 Knockdown %: not done |
ERBB2: No Change |
Link |
Link |
Impaired unique Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
ACTB Aliases: PS1TP5BP1 Entrez Gene: 60 siRNA catalog |
actin, beta mRNA: NM_001101 Library: MAR |
Link |
Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00021439 | ACTB | ACTIN, CYTOPLASMIC 1. | 997 | 59 | 1875 | 79 | 1.88 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00021439 | ACTB | ACTIN, CYTOPLASMIC 1. | 997 | 1875 | 1.88 | nucleus |
| IPI00021439 | ACTB | ACTIN, CYTOPLASMIC 1. | 997 | 1875 | 1.88 | cytoskeleton |
| IPI00021439 | ACTB | ACTIN, CYTOPLASMIC 1. | 997 | 1875 | 1.88 | cytosol |
Discovery» MTLn3 invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Actb Entrez Gene: 11461 |
mRNA: C78835 UniGene: Mm.391967 |
Actin, beta, cytoplasmic | 2.9 |
Pathways

