VCL Orthologs
Aliases: Metavinculin, vinculin, VCL, RP11-178G16.3, CMD1W, MVCL
Pfam Domain Structure
From VCL (human) - phosphorylation sites added via direct mapping
Members of the VCL ortholog set
| Human | Mouse | Rat | Chicken | Fly | Worm | |
| CMKB | VCL | Vcl | Vcl | VCL | - | - |
| Entrez Gene | 7414 | 22330 | 305679 | 396422 | - | - |
| Uniprot | VINC_HUMAN | VINC_MOUSE | VINC_RAT | VINC_CHICK | - | - |
| OMIM | 193065 | - | - | - | - | - |
| Reagents | - | 4 available | - | 3 available | - | - |
| Results | 4 available | 2 available | - | - | - | - |
References
- Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC. Structural basis for vinculin activation at sites of cell adhesion. Nature 2004; 430:583-6 (PubMed)
- Bois PR, O'Hara BP, Nietlispach D, Kirkpatrick J, Izard T. The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin. J. Biol. Chem. 2006; 281:7228-36 (PubMed)
- Burridge K, Mangeat P. An interaction between vinculin and talin. Nature 1984; 308:744-6 (PubMed)
- Critchley DR. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 2004; 32:831-6 (PubMed)
- del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP. Stretching single talin rod molecules activates vinculin binding. Science 2009; 323:638-41 (PubMed)
- Gilmore AP, Burridge K. Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate. Nature 1996; 381:531-5 (PubMed)
- Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature 2010; 466:263-6 (PubMed)
- Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
- Mierke CT, Kollmannsberger P, Zitterbart DP, Diez G, Koch TM, Marg S, Ziegler WH, Goldmann WH, Fabry B. Vinculin facilitates cell invasion into three-dimensional collagen matrices. J. Biol. Chem. 2010; 285:13121-30 (PubMed)
- Nayal A, Webb DJ, Horwitz AF. Talin: an emerging focal point of adhesion dynamics. Curr. Opin. Cell Biol. 2004; 16:94-8 (PubMed)
- Spinardi L, Marchisio PC. Podosomes as smart regulators of cellular adhesion. Eur. J. Cell Biol. 2006; 85:191-4 (PubMed)
- Ziegler WH, Liddington RC, Critchley DR. The structure and regulation of vinculin. Trends Cell Biol. 2006; 16:453-60 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| VCL | none | phosphorylation site | Vinculin | Ectopic expression in HEK cells | 2 phospho-sites in 92.1% coverage of 1062 aa | Parsons |
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| VCL | none | structure | Alpha-actinin / beta1-integrin - Vinculin 1-258 complex | 2D crystallization on lipid monolayers | completed | Taylor | Kelly et al. |
| VCL | none | structure | Vinculin (full length) | X-Ray crystallography | data available | Liddington | Bakolitsa et al., Bakolitsa et al., Saunders et al., Ziegler et al. |
| VCL | none | structure | Vinculin-tail actin | EM, tomography and docking | completed | Hanein | Janssen et al. |
| VCL | none | structure | Meta-vinculin C-terminal domain | X-Ray crystallography, NMR | completed | Liddington | Bakolitsa et al. |
Discovery» MTLn3 invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Vcl Entrez Gene: 22330 |
mRNA: AA275245 UniGene: Mm.279361 |
Vinculin | 2.2 |
Discovery» PyMT invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Vcl Entrez Gene: 22330 |
mRNA: AA275245 UniGene: Mm.279361 |
Vinculin | 18.06 |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
VCL Aliases: MVCL Entrez Gene: 7414 siRNA catalog |
vinculin mRNA: NM_003373 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: No change Avg area: 1.34 Avg Alamar: 0.97 Knockdown %: not done |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
VCL Aliases: MVCL Entrez Gene: 7414 siRNA catalog |
vinculin mRNA: NM_003373 Library: MAR |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00291175 | VCL | VINCULIN ISOFORM VCL . | 94 | 34 | 156 | 45 | 1.66 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00291175 | VCL | VINCULIN ISOFORM VCL . | 94 | 156 | 1.66 | cytoskeleton |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| VCL | mouse | Vinculin | - |
Death by E10, with embryos displaying 30-40% reduction in size, lack of midline fusion of the rostral neural tube and developmental defects in heart, somites and limbs. | completed |
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Xu et al. |
| VCL | mouse | Vinculin - F9 embryonal carcinoma cells | - |
Normal adherence to fibronectin, reduced spreading, increased amounts of alpha-actinin, talin and paxillin at focal adhesions. | completed |
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|
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Volberg et al. |
| VCL | mouse | Vinculin - F9 embryonal carcinoma cells | - |
Altered cell morphology and motility. Absence of stress fibers. | completed |
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Goldmann et al. |
| VCL | mouse | Vinculin - embryonic fibroblasts | - |
Reduced adhesion to fibronectin, vitronectin, laminin and collagen and increased migration rates over these substrates. | completed |
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Xu et al. |
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| VCL | chicken | Vinculin | pET15b-VD1 | 1-258 | Vinculin Head domain (Y100E mutant) | available | Liddington |
| VCL | chicken | Vinculin | pET15b-VD1 | 1-258 | Vinculin -head domain 1 | - |
Liddington |
| VCL | chicken | Vinculin | pET15b-VD1 | 879-1066 | Vinculin Tail (Y1064E mutant) | available | Liddington |
Biosensors » activity page
| Ortholog | Species | Name | Type | Source | Publications |
|---|---|---|---|---|---|
| VCL | none | Vinculin | Intramolecular FRET between FPs - Insertion of a FRET pair in vinculin |
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Chen et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Pathways