Grb2 mouse: growth factor receptor bound protein 2
Aliases: Adapter protein GRB2, SH2/SH3 adapter GRB2, growth factor receptor bound protein 2, Ash, Grb2, RP23-28I4.1, AA408164
Pfam Domain Structure
Protein Overview
| Official Gene Name | Grb2 (M. musculus) |
| RefSeq Protein Name | growth factor receptor bound protein 2 |
| UniProt Gene Symbol | GRB2_MOUSE |
| UniProt Name | Growth factor receptor-bound protein 2 |
References
| 1). Dharmawardana PG, Peruzzi B, Giubellino A, Burke TR, Bottaro DP. Molecular targeting of growth factor receptor-bound 2 (Grb2) as an anti-cancer strategy Anticancer Drugs 2006 17:13-20 (PubMed) | ||
| 2). Feller SM, Lewitzky M. Potential disease targets for drugs that disrupt protein-- protein interactions of Grb2 and Crk family adaptors Curr. Pharm. Des. 2006 12:529-48 (PubMed) | ||
| 3). Johannessen LE, Pedersen NM, Pedersen KW, Madshus IH, Stang E. Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits Mol. Cell. Biol. 2006 26:389-401 (PubMed) | ||
| 4). Natarajan K, Berk BC. Crosstalk coregulation mechanisms of G protein-coupled receptors and receptor tyrosine kinases Methods Mol. Biol. 2006 332:51-77 (PubMed) | ||
| 5). Sármay G, Angyal A, Kertész A, Maus M, Medgyesi D. The multiple function of Grb2 associated binder (Gab) adaptor/scaffolding protein in immune cell signaling Immunol. Lett. 2006 104:76-82 (PubMed) | ||
| 6). Zarich N, Oliva JL, Martínez N, Jorge R, Ballester A, Gutiérrez-Eisman S, García-Vargas S, Rojas JM. Grb2 is a negative modulator of the intrinsic Ras-GEF activity of hSos1 Mol. Biol. Cell 2006 17:3591-7 (PubMed) | ||
Consortium Results & Data
Discovery» Drosophila Migration Genes
| Gene | Name | Function | Cell Type | Phenotypes | Reagents/ Alleles | Reference |
| drk Entrez: CG6033 Flybase: FBgn0004638 | Downstream of receptor kinase | signal transduction, adaptor | embryonic hemocyte | Loss of function: no zygotic phenotype | not clear what kind of allele: drkDP2 | Cho 2002 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
| drk Flybase Entrez CMCKB | downstream of receptor kinase | Mig: 83.8 Non-Mig: 51.1 | 1.63990 | 153747_at |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | Secondary screen | Wound Image | Morphology | Time-lapse | |
| GRB2 Aliases: ASH, Grb3-3, MST084, MSTP084, EGFRBP-GRB2 Entrez Gene: 2885 siRNA catalog | growth factor receptor-bound protein 2 mRNA: NM_002086 Library: MAR Classification: adaptor Focal Adhesion Related: Yes | Final bin: Low Alamar - MC SMARTpool bin: Low Alamar Avg area: 4.05 Avg Alamar: 0.51 Knockdown %: not done | ERBB2: No Change BCL2: Low Alamar |  Link |  Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
| GRB2 Aliases: ASH, Grb3-3, MST084, MSTP084, EGFRBP-GRB2 Entrez Gene: 2885 siRNA catalog | growth factor receptor-bound protein 2 mRNA: NM_002086 Library: MAR |  Link |  Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00021327 | GRB2 | GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2. | 3 | 2 | 3 | 2 | 1.00 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00021327 | GRB2 | GROWTH FACTOR RECEPTOR-BOUND PROTEIN 2. | 3 | 3 | 1 | cytosol |
Discovery» Genes that increase migratory behavior in MCF-10A cells
| Protein | Protein Class | BC-1000 cDNA | mcf7/ mcf10a | mcf7/ mcf10a-csf1r | mcf7/ mcf-10a-csf1r_csf | mcf7/ mcf10a-erbb2 |
| GRB2 Entrez GeneID: 2885 CMKB Orthologs | Signal transduction |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Comments | Source | Publications |
|---|---|---|---|---|---|---|---|---|---|
| GRB2 | mouse | Grb2 - hypomorph | - |
Perinatal death due to cleft palate. | completed |
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Saxton et al. |
| GRB2 | mouse | Grb2 | - |
Embryonic lethality at E4. Failure in endoderm differentiation. Chimeric embryos show that Grb2-null cells do not contribute to the epiblast. | completed |
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Cheng et al., Saxton et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| NP_032189 | NM_008163 | Q60631-1 |
bold indicates the primary isoform
Interactions (from human)
| Source | Target | |||
|---|---|---|---|---|
| Name | Function | Interaction | Name | Function |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | INPP5D (Adhesome) | PtdInsPhosphatase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | IRS1 (Adhesome) | Adapter |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PAK1 (Adhesome) | SerThrKinase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PIK3CA (Adhesome) | PtdInsKinase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PLCG1 (Adhesome) | PtdInsKinase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PTK2B (Adhesome) | TyrKinase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PTPN1 (Adhesome) | TyrPhosphatase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PTPN11 (Adhesome) | TyrPhosphatase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PTPN12 (Adhesome) | TyrPhosphatase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PTPN6 (Adhesome) | TyrPhosphatase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | PTPRA (Adhesome) | TyrPhosphatase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | SHC1 (Adhesome) | Adapter |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | SOS1 (Adhesome) | RasGEF |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | SYK (Adhesome) | TyrKinase |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | TUBA1B (Adhesome) | Cytoskeletal |
| GRB2 (Adhesome) | Adapter | Binding (PubMed) | VAV1 (Adhesome) | GEF |
| ADAM12 (Adhesome) | Metalloproteinase | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| CBL (Adhesome) | E3ligase | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| CRK (Adhesome) | Cytoskeletal | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| DNM2 (Adhesome) | GTPase | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| GAB1 (Adhesome) | Adapter | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| JUB (Adhesome) | Cytoskeletal | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| PTK2 (Adhesome) | TyrKinase | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| RAPGEF1 (Adhesome) | GEF | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| SH3KBP1 (Adhesome) | Adapter | Binding (PubMed) | GRB2 (Adhesome) | Adapter |
| SRC (Adhesome) | TyrKinase | Activation (PubMed) | GRB2 (Adhesome) | Adapter |
Interaction information is courtesy of the Adhesome FA Network.
